Anti-viral HBV Studies

Worldwide, there are more than 350 million chronic carriers of HBV of which approximately one million die each year from complications of the disease. This makes chronic HBV one of the ten most common causes of death. In the United States, approximately 1.25 million people are believed to have chronic HBV and an estimated 200,000 people become newly infected each year. Hepatitis B is the leading cause of liver cancer. Approximately 4,000 to 5,000 Americans die each year from hepatitis-B related chronic liver disease or liver cancer.

Woodchuck hepatitis virus (WHV), is a member of the Hepadnaviridae, and is closely related to HBV in its virus genetic organization and mechanism of replication. Natural infection of woodchucks produces chronic liver disease and primary hepatocellular carcinoma (HCC). As in humans, animals infected as newborns develop chronic antigenemia at higher rates than do young adults. The predictable course of experimental WHV infection leading to liver disease in woodchucks makes them an ideal animal model in which to study the natural history of hepadenavirus and to develop effective anti-viral strategies. In fact, recent research comparing the results of the effect of nucleoside analogs in woodchucks with their effect in humans, demonstrates that the woodchuck is a predictive model for antiviral therapy against HBV in humans.

Since 1990, Northeastern Wildlife has been completing antiviral studies in woodchucks at our facility. We offer a broad range of contract research services and years of experience to meet your pre-clinical needs. We can also arrange consulting agreements with investigators who are specialists in Woodchuck Hepatitis Virus (WHV) infection if required.

Northeastern Wildlife's contract research services are very cost effective due to our low overhead. We have the most experienced technicians available in the WHV research field who also happen to be the owners! Protocols are performed correctly as our reputations and livelihood are at stake. You don't have to worry about hurried data collection by disinterested employees or work study students whose only interest is in leaving early for the weekend!

Some of Northeastern Wildlife's HBV contract research services include the following:

1) Protocol development and implementation.

2) Pharmacokinetic studies

3) Long term studies which assess anti-viral effect on Hepatocellular Carcinoma (HCC)

4) Installation of vascular access ports for long term I.V. and I.P. administrations

5) Oral dosing of animals

6) Full wedge and needle liver biopsy surgical services

7) Blood collection, harvesting, storage and shipping of samples

8) Necropsy services

9) A full range of diagnostic services such as serological profiles which include small animal panels, coagulation panels, histology, hemogram routines etc.

10) WHV virological assays which include serological markers of WHV infection, Dot Blot assays, Real Time PCR assays, Southern Blot hybridization, Viral Proteins, In Situ Hybridization, Immunohistochemistry, PCR amplification sequencing and cloning etc.

If you have an antiviral which shows activity against HBV, contact us. We will submit an estimate for your consideration.

Hibernation Studies

In 1990, we constructed a temperature controlled hibernaculum to perform woodchuck hibernation studies. Current hibernation projects focus on the role of hibernation triggers which are present in hibernating woodchuck plasma. These triggers have been shown to extend organ transplant time and possess anti-clotting capabilities. Other projects involve the study of cardiac function during and after hibernation. Contact us for pricing and availablity of the hibernaculum for your hibernation studies.

Non-traditional Animal Model Development

Northeastern Wildlife has supplied several species of wildlife that exhibit similarities to specific human diseases. Contract with us to produce your animal model or inquire about our reduced research fees offered during animal model development.

Large Animal Capabilities

Are you faced with high per diem rates and limited space for your large research animals? We can help! Northeastern Wildlife is located on 30 acres in northern Idaho. We have space to meet your large animal needs!